Coding
CesA

Part:BBa_K3098001

Designed by: Yiru Shen   Group: iGEM19_WHU-China   (2019-09-23)


GxCesA

GxCesA protein is a GT-2 glycosyltransferase containing transmembrane helices and the catalytic subunit of cellulose synthase. The first cellulose synthase gene was identified in Gluconacetobacter xylinus (formly Acetobacter xylinum). In this bacterium, four genes are clustered in one operon, namely, gxcesA, gxcesB, gxcesC and gxcesD (formly bcsA, bcsB, bcsC and bcsD). The gene of cesA is found in all living cellulose-producing organisms, whereas the other genes are specific to bacterial cellulose synthase. In the case of bacteria, the CesA and CesB proteins represent the minimum subunit requirements for cellulose synthesis.

This part cannot work alone. See Cellulose Synthase (Part:BBa_K3098005).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 580
    Illegal PstI site found at 742
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 580
    Illegal PstI site found at 742
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 580
    Illegal BglII site found at 1142
    Illegal XhoI site found at 232
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 580
    Illegal PstI site found at 742
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 580
    Illegal PstI site found at 742
    Illegal NgoMIV site found at 212
    Illegal AgeI site found at 2193
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1717


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Parameters
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